PCR Molecular Technician

Department: Laboratory / Molecular Diagnostics
Reports To: Laboratory Supervisor or Molecular Lab Manager
Employment Type: Full-Time

Position Summary:

The PCR Molecular Technician is responsible for performing molecular diagnostic testing
using Polymerase Chain Reaction (PCR) techniques to detect and quantify nucleic acids in
clinical, environmental, or research samples. This role ensures accurate and timely results
while adhering to strict quality control and regulatory standards.

Key Responsibilities:

 Sample Preparation: Receive, log, and prepare biological samples for PCR testing
following established protocols.
 PCR/Clinical Testing: Perform DNA/RNA extraction, PCR amplification, detection
using real-time PCR instruments and related technologies and other clinical testing
assigned by the supervisor.
Quality Control: Implement and monitor quality assurance measures, including
running controls and calibrating equipment.
Data Analysis: Interpret PCR results, validate findings, and enter data into
Laboratory Information Management Systems (LIMS).
 Compliance: Maintain compliance with CLIA, CAP, ISO, and other regulatory
standards.
 Documentation: Accurately record all procedures, observations, and results in
accordance with laboratory SOPs.
 Equipment Maintenance: Perform routine maintenance and troubleshooting of
PCR instruments and related equipment.
 Safety: Follow all biosafety and chemical safety guidelines to ensure a safe working
environment.

Qualifications:

 Education: Associate or Bachelor’s degree in Molecular Biology, Biotechnology,
Medical Laboratory Science, or related field.
 Experience:
o 1–2 years of experience in PCR-based testing (clinical or research setting
preferred).
o Familiarity with nucleic acid extraction and real-time PCR platforms.

 Skills:

  •  Strong attention to detail and ability to follow protocols precisely.
  •  Proficiency in data analysis and laboratory software.
  •  Excellent organizational and communication skills.

Preferred Qualifications:

  •  Experience with high-throughput PCR systems.
  •  Knowledge of next-generation sequencing (NGS) workflows.
  •  Certification as a Molecular Biology Technologist (e.g., ASCP MB).

Work Environment:

  •  Laboratory setting with exposure to biological samples and chemicals.
  • Requires standing for extended periods and occasional lifting of up to 25 lbs.